SIK1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

SIK1 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240961H3

Kat-Nr. : 83897-4-PBS

Synonyme

240961H3, EC:2.7.11.1, MSK, salt inducible kinase 1, Salt-inducible kinase 1

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  WB analysis using 83897-4-RR (same clone as 83897-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83897-4-RR (SIK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83897-4-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83897-4-RR (same clone as 83897-4-PBS)

  WB result of SIK1 antibody (83897-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIK1 transfected HeLa cells. This data was developed using the same antibody clone with 83897-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 83897-4-RR (same clone as 83897-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 83897-4-RR (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83897-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83897-4-RR (same clone as 83897-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SIK1 antibody (83897-4-RR, Clone: 240961H3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83897-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00838-2

  Cytometric bead array standard curve of MP00838-2, SIK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83897-4-PBS. Detection antibody: 83897-2-PBS. Standard: Ag28481. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 83897-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83897-4-PBS against Human SIK1 was performed. The affinity constant is 36.2 pM.

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  Affinity and Kinetic Characterization of 83897-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83897-4-RR against Human SIK1 were performed. The affinity constant is 0.382 nM.

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Geprüfte Anwendungen

Produktinformation

83897-4-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA SIK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SIK1 fusion protein Ag28481
• Vollständiger Name: salt-inducible kinase 1
• Berechnetes Molekulargewicht: 783 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC038504
• Gene symbol: SIK1
• Gene ID (NCBI): 150094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832).