SIM2 Polyklonaler Antikörper

SIM2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21069-1-PBS

Synonyme

Class E basic helix-loop-helix protein 15, SIM, Single minded homolog 2, Single-minded homolog 2

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  WB analysis using 21069-1-AP (same clone as 21069-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

  IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21069-1-AP (same clone as 21069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21069-1-AP (SIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21069-1-PBS bindet in WB, IHC, IP, Indirect ELISA SIM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIM2 fusion protein Ag15204
• Vollständiger Name: single-minded homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 667 aa, 73 kDa
• Beobachtetes Molekulargewicht: 60-64 kDa
• GenBank-Zugangsnummer: BC110444
• Gene symbol: SIM2
• Gene ID (NCBI): 6493
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.