SIRP Alpha/CD172a Polyklonaler Antikörper

SIRP Alpha/CD172a Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31485-1-AP

Synonyme

SIRP Alpha, SIRPA, MFR, Inhibitory receptor SHPS-1, CD172a

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  WB analysis using 31485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 31485-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 31485-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (31485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of THP-1 using 31485-1-AP

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, NIH/3T3-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen
• Erfolgreiche Detektion in FC (Intra): THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31485-1-AP bindet in WB, IF/ICC, FC (Intra), ELISA SIRP Alpha/CD172a und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 65-100 kDa
• GenBank-Zugangsnummer: NM_080792
• Gene symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).