SIRP Alpha/CD172a Rekombinanter Antikörper

SIRP Alpha/CD172a Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240789C6

Kat-Nr. : 83729-5-RR

Synonyme

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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  WB analysis of U-937 using 83729-5-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 83729-5-RR (SIRP Alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 83729-5-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-5-RR, Clone: 240789C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83729-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83729-5-RR against Human SIRP Alpha were performed. The affinity constant is 0.497 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83729-5-RR bindet in WB, IF/ICC, ELISA SIRP Alpha/CD172a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: NM_080792
• Gene symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).