SIRP Alpha/CD172a Rekombinanter Antikörper

SIRP Alpha/CD172a Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241794D9

Kat-Nr. : 84430-2-RR

Synonyme

Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A, Brain Ig-like molecule with tyrosine-based activation motifs, Bit

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  WB analysis using 84430-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84430-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84430-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84430-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 84430-2-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SIRP alpha/CD172a antibody (84430-2-RR, Clone: 241794D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84430-2-RR bindet in WB, IHC, IF/ICC, ELISA SIRP Alpha/CD172a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FusionProtein
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 90-140 kDa
• GenBank-Zugangsnummer: NM_007547.4
• Gene symbol: Sirpa
• Gene ID (NCBI): 19261
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).