SIRP Alpha/CD172a Rekombinanter Antikörper

SIRP Alpha/CD172a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241794D9

Kat-Nr. : 98225-1-PBS

Synonyme

241794D9, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a

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  FC experiment of mouse bone marrow cells using 98225-1-RR (same clone as 98225-1-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were not fixed. This data was developed using the same antibody clone with 98225-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse bone marrow cells using 98225-1-RR (same clone as 98225-1-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98225-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98225-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98225-1-RR against Mouse SIRP Alpha/CD172a were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98225-1-PBS bindet in FC SIRP Alpha/CD172a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SIRP Alpha/CD172a fusion protein Eg1520
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: NM_007547.4
• Gene symbol: Sirpa
• Gene ID (NCBI): 19261
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).