SIRP Alpha/CD172a Rekombinanter Antikörper

SIRP Alpha/CD172a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241794D9

Kat-Nr. : CL647-98225

Synonyme

Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A, Brain Ig-like molecule with tyrosine-based activation motifs, Bit

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  FC experiment of mouse bone marrow cells using CL647-98225

  1x10^6 mouse bone marrow cells were surface stained with FITC Plus Anti-Mouse CD11b (M1/70) (FITC-65055, Clone: M1/70), and 0.25 ug CoraLite® Plus 647 Anti-Mouse SIRP Alpha/CD172a Rabbit RecAb (CL647-98225, Clone: 241794D9) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL647-98225

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse SIRP Alpha/CD172a Rabbit RecAb (CL647-98225, Clone: 241794D9) (red) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98225 bindet in FC SIRP Alpha/CD172a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: NM_007547.4
• Gene symbol: Sirpa
• Gene ID (NCBI): 19261
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).