SIRP Alpha/CD172a Monoklonaler Antikörper

SIRP Alpha/CD172a Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F8H3

Kat-Nr. : 67337-1-Ig

Synonyme

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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  WB analysis using 67337-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 67337-1-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67337-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 67337-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 67337-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, HL-60-Zellen, Maushirngewebe, Kaninchenhirngewebe, Rattenhirngewebe, SW480-Zellen, THP-1-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Cerebellum-Gewebe, humanes Lebergewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67337-1-Ig bindet in WB, IHC, ELISA SIRP Alpha/CD172a und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIRP Alpha/CD172a fusion protein Ag6361
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC033092
• Gene symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.