SIRP Alpha/CD172a Polyklonaler Antikörper

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• KD/KO Validated

SIRP Alpha/CD172a Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (2)

Kat-Nr. : 14482-1-AP

Synonyme

SIRP Alpha, SIRPA, BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A

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  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 14482-1-AP

  WB result of SIRP Alpha/CD172a antibody (14482-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRP Alpha/CD172a transfected U-937 cells.

• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 14482-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 14482-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 14482-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (14482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, Maus-Cerebellum-Gewebe, PC-12-Zellen, Ratten-Cerebellum-Gewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

14482-1-AP bindet in WB, IHC, IF/ICC, ELISA SIRP Alpha/CD172a und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRP Alpha/CD172a fusion protein Ag5865
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC033092
• Gene symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

Publikationen

Species	Application	Title
mouse	WB	Cell Prolif; Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling; Authors - Qing Tu
mouse	WB	Acta Biochim Biophys Sin (Shanghai); Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6.; Authors - Kejia Zhao
human	WB	J Obstet Gynaecol Res; Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages.; Authors - Qi Xie
human	WB	Biomedicines; Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis; Authors - Ying Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manuel (Verified Customer) (03-19-2025)

Works well.

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: THP-1

FH

Rebecca (Verified Customer) (05-27-2024)

Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC. Multiple bands observed. A bigger dilution of the antibody is probably needed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSC-CM

SIRP Alpha/CD172a Antibody Western Blot validation (1:1000 dilution) in hiPSC-CM (Cat no:14482-1-AP)