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SIRP Alpha/CD172a Rekombinanter Antikörper

SIRP Alpha/CD172a Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240789C6

Kat-Nr. : 83729-5-PBS

Synonyme

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

Filter:
  • Western Blot (WB) analysis of U-937 cells using SIRP Alpha/CD172a Recombinant antibody (83729-5-RR)

    WB analysis of U-937 using 83729-5-RR (same clone as 83729-5-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 83729-5-RR (SIRP Alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83729-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using SIRP Alpha/CD172a Recombinant antibody (83729-5-RR)

    IF Staining of THP-1 using 83729-5-RR (same clone as 83729-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-5-RR, Clone: 240789C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83729-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83729-5-RR

    Affinity and Kinetic Characterization of 83729-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83729-5-RR against Human SIRP Alpha were performed. The affinity constant is 0.497 nM.

Geprüfte Anwendungen

Produktinformation

83729-5-PBS bindet in WB, IF/ICC, ELISA SIRP Alpha/CD172a und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name signal-regulatory protein alpha
Berechnetes Molekulargewicht55 kDa
Beobachtetes Molekulargewicht 75-85 kDa
GenBank-ZugangsnummerNM_080792
Gene symbol SIRP Alpha
Gene ID (NCBI) 140885
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).