SIRT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SIRT1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

291

Rezensionen (2)

Kat-Nr. : 13161-1-AP

Synonyme

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

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Galerie der Validierungsdaten

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  WB analysis using 13161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13161-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 13161-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (13161-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 13161-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (13161-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13161-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13161-1-AP (SIRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 31 publications below
• WB: See 268 publications below
• IHC: See 40 publications below
• IF: See 35 publications below
• IP: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 7 publications below

Produktinformation

13161-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA SIRT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Rind, Zebrafisch, Ziege, Riesenscheibenbrasse (Megalobrama amblycephala)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRT1 fusion protein Ag3808
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 747 aa, 82 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa, 80-85 kDa
• GenBank-Zugangsnummer: BC012499
• Gene symbol: SIRT1
• Gene ID (NCBI): 23411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttles between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.

Publikationen

Species	Application	Title
	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
	WB,IF	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu ; KD Validated
	WB,IHC	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
	IF	J Pineal Res; Melatonin enhances mitochondrial biogenesis and protects against rotenone-induced mitochondrial deficiency in early porcine embryos.; Authors - Ying-Jie Niu
human	WB,IP	Cell Rep Med; Decoupling NAD+ metabolic dependency in chondrosarcoma by targeting the SIRT1-HIF-2α axis; Authors - Jooyeon Suh
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Uxoa (Verified Customer) (02-19-2020)

Non nuclear signal of SIRT1 in human fibroblast. Cells fixed with 4% PFA 15 min. Ab dilution 1:100 in PBST (0.1% triton)+10% GS. O/N 4ºC incubation. Secondary ab: Goat anti-rabbit alexafluor 555 1:500 in PBST + 10% GS 1h RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human fibroblasts

SIRT1 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:13161-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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