SIRT1 Rekombinanter Antikörper

SIRT1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

4F14

Kat-Nr. : 82790-2-RR

Synonyme

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

82790-2-RR bindet in WB, ELISA SIRT1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SIRT1 fusion protein Ag3808
Vollständiger Name sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Berechnetes Molekulargewicht 747 aa, 82 kDa
Beobachtetes Molekulargewicht110-130 kDa
GenBank-ZugangsnummerBC012499
Gene symbol SIRT1
Gene ID (NCBI) 23411
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Protokolle

Produktspezifische Protokolle
WB protocol for SIRT1 antibody 82790-2-RRProtokoll herunterladen
Standard-Protokolle
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