SIRT1 Multi-Recombinanter Antikörper

SIRT1 Multi-Recombinant Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : RMX00009

Synonyme

EC:2.3.1.-, EC:2.3.1.286, hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1

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  WB analysis using RMX00009

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00009 (SIRT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using RMX00009

  IP result of anti-SIRT1 (IP:RMX00009, 4ug; Detection:RMX00009 1:4000) with HeLa cells lysate 1200 ug.

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  IHC staining of human colon cancer using RMX00009

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using RMX00009

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using RMX00009

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using RMX00009

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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  IF Staining of A431 using RMX00009

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

RMX00009 bindet in WB, IHC, IF/ICC, IP, ELISA SIRT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Multi-Recombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 747 aa, 82 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: BC012499
• Gene symbol: SIRT1
• Gene ID (NCBI): 23411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT1, also named SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein containing one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttle between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.