SIRT2 Polyklonaler Antikörper

SIRT2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 15345-1-AP

Synonyme

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

---

Galerie der Validierungsdaten

• 

  WB analysis of human brain using 15345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

15345-1-AP bindet in WB, IHC,ELISA SIRT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRT2 fusion protein Ag7556
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 37-45 kDa
• GenBank-Zugangsnummer: BC003547
• Gene symbol: SIRT2
• Gene ID (NCBI): 22933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. This antibody is a rabbit polyclonal antibody raised against the N-terminal 352 residues of human SIR2 protein.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance.; Authors - Ming He ; KO Validated