SIRT3 Rekombinanter Antikörper

SIRT3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2P6

Kat-Nr. : 82210-1-PBS

Synonyme

EC:2.3.1.286, hSIRT3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3

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  WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82210-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82210-1-RR against Human SIRT3 were performed. The affinity constant is 0.261 nM.

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Geprüfte Anwendungen

Produktinformation

82210-1-PBS bindet in WB, IHC, Indirect ELISA SIRT3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SIRT3 fusion protein Ag26280
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC001042
• Gene symbol: SIRT3
• Gene ID (NCBI): 23410
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.