SIRT4 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

SIRT4 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

CloneNo.

1D5A2

Kat-Nr. : 66543-1-Ig

Synonyme

SIR2 like protein 4, SIR2L4, SIRT4

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 66543-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66543-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66543-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66543-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:80000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

66543-1-Ig bindet in WB, IHC, IF, ELISA SIRT4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIRT4 fusion protein Ag17347
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC109319
• Gene symbol: SIRT4
• Gene ID (NCBI): 23409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.; Authors - Shuai Leng ; KD Validated
human	WB	Cancer Lett; SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.; Authors - Mengqi Liu
human	WB	Int Immunopharmacol; Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma; Authors - Jiayuan Liang
human	WB,IHC,IF	Lab Invest; Targeting SIRT4/TET2 Signaling Alleviates Human Keratinocyte Senescence by Reducing 5-hmC Loss; Authors - Yi Yi
human	WB	Dis Model Mech; Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.; Authors - Leonie Drews
human	WB	Sci Rep; Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.; Authors - Kalaiselvi Sivalingam