SIRT4 Monoklonaler Antikörper

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• KD/KO Validated

SIRT4 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5A2

Kat-Nr. : 66543-1-PBS

Synonyme

EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4

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• 

  WB analysis of HeLa using 66543-1-Ig (same clone as 66543-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66543-1-Ig (same clone as 66543-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66543-1-Ig (same clone as 66543-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66543-1-Ig (same clone as 66543-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66543-1-PBS bindet in WB, Indirect ELISA SIRT4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIRT4 fusion protein Ag17347
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC109319
• Gene symbol: SIRT4
• Gene ID (NCBI): 23409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.