SIRT5 Polyklonaler Antikörper

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• KD/KO Validated

SIRT5 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Kat-Nr. : 15122-1-AP

Synonyme

SIR2 like protein 5, SIR2L5, SIRT5

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Galerie der Validierungsdaten

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  WB analysis using 15122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15122-1-AP

  IP Result of anti-SIRT5 (IP:15122-1-AP, 4ug; Detection:15122-1-AP 1:500) with mouse liver tissue lysate 6000ug.

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  IF Staining of HeLa using 15122-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15122-1-AP (SIRT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, Mausherzgewebe, Mauslebergewebe, Rattenherzgewebe, Rattenlebergewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 32 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

15122-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA SIRT5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRT5 fusion protein Ag7195
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000126
• Gene symbol: SIRT5
• Gene ID (NCBI): 23408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
rat	WB,IF,CoIP	Exp Mol Med; SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis; Authors - Jianxin Mao ; KD Validated
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma ; KD Validated
mouse	WB	Diabetes; Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.; Authors - Hua Qu ; KD Validated