SIRT5 Monoklonaler Antikörper

SIRT5 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1F2B2

Kat-Nr. : 67257-1-Ig

Synonyme

1F2B2, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5

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  WB analysis of HeLa using 67257-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67257-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 67257-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 67257-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67257-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:4800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, K-562-Zellen, L02-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

67257-1-Ig bindet in WB, IHC, IF, IP, ELISA SIRT5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIRT5 fusion protein Ag7743
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000126
• Gene symbol: SIRT5
• Gene ID (NCBI): 23408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.

Publikationen

Species	Application	Title
human	WB,IP,IF	Adv Sci (Weinh); SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma; Authors - Qifan Hu
human	WB	EMBO J; TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.; Authors - Qichao Huang
	WB,IF	Inflammation; Moxibustion Alleviates Inflammation via SIRT5-mediated Post-translational Modification and Macrophage Polarization; Authors - Chuan-Yi Zuo
bovine	WB,IP	Cell Signal; SIRT5-mediated GLS and GDH desuccinylation attenuates the autophagy of bovine mammary epithelial cells induced by ammonia; Authors - Hanlin Yang ; KO Validated
human	WB	Gynecol Endocrinol; SIRT5 regulates granulosa cell proliferation and apoptosis in polycystic ovarian syndrome via desuccinylation of GLI1; Authors - Yiwen Zhang
	WB	Genes Dis; SIRT5 promotes the osteo-inductive potential of BMP9 by stabilizing the HIF-1α protein in mouse embryonic fibroblasts; Authors - Lu Liu