SIRT5 Monoklonaler Antikörper

SIRT5 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2B2

Kat-Nr. : 67257-1-PBS

Synonyme

1F2B2, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5

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  WB analysis of HeLa using 67257-1-Ig (same clone as 67257-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67257-1-Ig (same clone as 67257-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 67257-1-Ig (same clone as 67257-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67257-1-Ig (same clone as 67257-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67257-1-Ig (same clone as 67257-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:4800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67257-1-PBS bindet in WB, IHC, Indirect ELISA SIRT5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIRT5 fusion protein Ag7743
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000126
• Gene symbol: SIRT5
• Gene ID (NCBI): 23408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.