SIRT6 Polyklonaler Antikörper

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• KD/KO Validated

SIRT6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

39

Kat-Nr. : 13572-1-AP

Synonyme

SIR2 like protein 6, SIR2L6, SIRT6

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Galerie der Validierungsdaten

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  WB analysis using 13572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13572-1-AP (SIRT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13572-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13572-1-AP (SIRT6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 13572-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13572-1-AP (SIRT6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13572-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13572-1-AP (SIRT6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 13572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13572-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HepG2-Zellen, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 27 publications below
• IHC: See 12 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13572-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA SIRT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRT6 fusion protein Ag4469
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC028220
• Gene symbol: SIRT6
• Gene ID (NCBI): 51548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889). This antibody is a rabbit polyclonal antibody raised against full length human SIRT6 antigen.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB	Redox Biol; A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.; Authors - Mengjie Xiao
human	WB,IF	Oncogene; Sirtuin-mediated deacetylation of hnRNP A1 suppresses glycolysis and growth in hepatocellular carcinoma.; Authors - Hao Yang
rat	WB,IF	Stem Cells; SIRT6 regulates osteogenic differentiation of rat bone marrow mesenchymal stem cells partially via suppressing the nuclear factor-kappa B signaling pathway.; Authors - Hualing Sun ; KD Validated
mouse	WB	Oxid Med Cell Longev; Total Sesquiterpene Glycosides from Loquat Leaves Ameliorate HFD-Induced Insulin Resistance by Modulating IRS-1/GLUT4, TRPV1, and SIRT6/Nrf2 Signaling Pathways.; Authors - Ruoyun Wu

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