SIRT6 Monoklonaler Antikörper

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• KD/KO Validated

SIRT6 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2H4G8

Kat-Nr. : 67510-1-Ig

Synonyme

2H4G8, EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, hSIRT6

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  WB analysis using 67510-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67510-1-Ig (SIRT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67510-1-Ig

  WB result of SIRT6 antibody (67510-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRT6 transfected Jurkat cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: JAR-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NCCIT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

67510-1-Ig bindet in WB, CoIP, ELISA SIRT6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIRT6 fusion protein Ag24103
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC028220
• Gene symbol: SIRT6
• Gene ID (NCBI): 51548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889).

Publikationen

Species	Application	Title
mouse	WB	Cell Prolif; 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.; Authors - Wenxing Cui
mouse	WB	ACS Omega; Harnessing the Therapeutic Potential of Ginsenoside Rd for Activating SIRT6 in Treating a Mouse Model of Nonalcoholic Fatty Liver Disease; Authors - Tianqi Cui
mouse	WB,CoIP	Mol Med; Microglia Sirt6 modulates the transcriptional activity of NRF2 to ameliorate high-fat diet-induced obesity; Authors - Xiaoxia Xiao
mouse	WB	J Orthop Res; Orientin inhibits inflammation in chondrocytes and attenuates osteoarthritis through Nrf2/NF-κB and SIRT6/NF-κB pathway; Authors - Weiyi Xia