SIRT7 Polyklonaler Antikörper

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• KD/KO Validated

SIRT7 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(28)

Kat-Nr. : 12994-1-AP

Synonyme

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

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  WB analysis using 12994-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12994-1-AP (SIRT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 12994-1-AP

  IP result of anti-SIRT7 (IP:12994-1-AP, 4ug; Detection:12994-1-AP 1:1000) with PC-3 cells lysate 960 ug.

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  IHC staining of mouse liver using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Mausnierengewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

12994-1-AP bindet in WB, IHC, IF, IP, ELISA SIRT7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRT7 fusion protein Ag3656
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Berechnetes Molekulargewicht: 400 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC017305
• Gene symbol: SIRT7
• Gene ID (NCBI): 51547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Aging Cell; Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells.; Authors - Weina Zhang
mouse	WB	Redox Biol; A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.; Authors - Mengjie Xiao
	WB	Oxid Med Cell Longev; SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway.; Authors - Kun-Lin Chen
rat	WB	PLoS Pathog; KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation; Authors - Jing Zhou