• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SIRT7 Polyklonaler Antikörper

SIRT7 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12994-1-PBS

Synonyme

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

Filter:
  • Western Blot (WB) analysis of various lysates using SIRT7 Polyclonal antibody (12994-1-AP)

    WB analysis using 12994-1-AP (same clone as 12994-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12994-1-AP (SIRT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using SIRT7 Polyclonal antibody (12994-1-AP)

    IP experiment of PC-3 using 12994-1-AP (same clone as 12994-1-PBS)

    IP result of anti-SIRT7 (IP:12994-1-AP, 4ug; Detection:12994-1-AP 1:1000) with PC-3 cells lysate 960 ug. This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SIRT7 Polyclonal antibody (12994-1-AP)

    IHC staining of mouse liver using 12994-1-AP (same clone as 12994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SIRT7 Polyclonal antibody (12994-1-AP)

    IHC staining of mouse liver using 12994-1-AP (same clone as 12994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12994-1-PBS bindet in WB, IHC, IP, Indirect ELISA SIRT7 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SIRT7 fusion protein Ag3656
Vollständiger Name sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
Berechnetes Molekulargewicht 400 aa, 45 kDa
Beobachtetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC017305
Gene symbol SIRT7
Gene ID (NCBI) 51547
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.