SIX1 Monoklonaler Antikörper

SIX1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D7D1

Kat-Nr. : 67741-1-PBS

Synonyme

2D7D1, BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1

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  WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67741-1-Ig (same clone as 67741-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67741-1-PBS bindet in WB, IF/ICC, ELISA SIX1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIX1 fusion protein Ag28570
• Vollständiger Name: SIX homeobox 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC008874
• Gene symbol: SIX1
• Gene ID (NCBI): 6495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.