SIX2 Monoklonaler Antikörper

SIX2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9G11

Kat-Nr. : 66347-1-PBS

Synonyme

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

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  WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

  Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66347-1-Ig (same clone as 66347-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66347-1-Ig (same clone as 66347-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66347-1-Ig (same clone as 66347-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66347-1-Ig (same clone as 66347-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 66347-1-Ig (same clone as 66347-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 66347-1-Ig (same clone as 66347-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIX2 antibody (66347-1-Ig, Clone: 1F9G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66347-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SIX2 und zeigt Reaktivität mit Hausschwein, human, Hund, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIX2 fusion protein Ag2124
• Vollständiger Name: SIX homeobox 2
• Berechnetes Molekulargewicht: 291 aa, 32 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC024033
• Gene symbol: SIX2
• Gene ID (NCBI): 10736
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].