SIX2 Monoklonaler Antikörper

SIX2 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Hund, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1F9G11

Kat-Nr. : CL594-66347

Synonyme

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

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  IF Staining of HeLa using CL594-66347

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SIX2 antibody (CL594-66347, Clone: 1F9G11 ) at dilution of 1:1200, CL488-Phalloidin (green).

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  FC experiment of C2C12 using CL594-66347

  1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:600-1:2400
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66347 bindet in IF/ICC, FC (Intra) SIX2 und zeigt Reaktivität mit Hausschwein, human, Hund, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIX2 fusion protein Ag2124
• Vollständiger Name: SIX homeobox 2
• Berechnetes Molekulargewicht: 291 aa, 32 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC024033
• Gene symbol: SIX2
• Gene ID (NCBI): 10736
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].