SIX4 Polyklonaler Antikörper

SIX4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21305-1-AP

Synonyme

AREC3, Homeobox protein SIX4, SIX homeobox 4

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  WB analysis using 21305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21305-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse tongue using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse salivary gland using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat salivary gland using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 21305-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIX4 antibody (21305-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, Mauszungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

21305-1-AP bindet in WB, IHC, IF/ICC, ELISA SIX4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIX4 fusion protein Ag15918
• Vollständiger Name: SIX homeobox 4
• Berechnetes Molekulargewicht: 781 aa, 83 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC098282
• Gene symbol: SIX4
• Gene ID (NCBI): 51804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Six (sine oculis) proteins are a family of homeodomain transcription factors that share a conserved DNA-binding domain and are human homologs of the Drosophila sine oculis (so) protein. SIX homeobox 4 (SIX4), also known as AREC3, belongs to the the Six family. Six4 contains one Six domain (which funtions as a homeobox DNA-binding motif) and shares 90% sequence similarity with its mouse counterpart, suggesting that both proteins have similar DNA-binding properties. SIX4 is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles.

Publikationen

Species	Application	Title
human	WB	EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
mouse	WB	iScience; RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis; Authors - Hannah J Wiedner
mouse	IF	Dev Biol; A cellular and molecular mosaic establishes growth and differentiation states for cranial sensory neurons.; Authors - Beverly A Karpinski
mouse,human	WB,IHC,IF	Int J Biol Sci; SIX4 Activation in Inflammatory Response Drives the Transformation of Colorectal Epithelium into Inflammation and Tumor via Feedback-Enhancing Inflammatory Signaling to Induce Tumor Stemness Signaling; Authors - Ziyan Zhang ; KD Validated
mouse	WB	Sci Rep; SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia; Authors - Zhonghua Xu