SKAP2 Monoklonaler Antikörper

SKAP2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G3G9

Kat-Nr. : 66367-1-Ig

Synonyme

PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 66367-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 66367-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66367-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66367-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66367-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 66367-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SKAP2 antibody (66367-1-Ig, Clone: 2G3G9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Hausschwein-Lebergewebe, humanes Milzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66367-1-Ig bindet in WB, IHC, IF, ELISA SKAP2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SKAP2 fusion protein Ag3615
• Vollständiger Name: src kinase associated phosphoprotein 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC036044
• Gene symbol: SKAP2
• Gene ID (NCBI): 8935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.