SKIL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SKIL Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19218-1-PBS

Synonyme

---

• 

  WB analysis using 19218-1-AP (same clone as 19218-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 19218-1-AP (same clone as 19218-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

19218-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SKIL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SKIL fusion protein Ag5282
• Vollständiger Name: SKI-like oncogene
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC059386
• Gene symbol: SKIL
• Gene ID (NCBI): 6498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.