SKIV2L Monoklonaler Antikörper

SKIV2L Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A9G2

Rezensionen (2)

Kat-Nr. : 68547-1-Ig

Synonyme

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

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  WB analysis using 68547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 68547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 68547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68547-1-Ig bindet in WB, IHC, ELISA SKIV2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SKIV2L fusion protein Ag33679
• Vollständiger Name: superkiller viralicidic activity 2-like (S. cerevisiae)
• Berechnetes Molekulargewicht: 1246 aa, 138 kDa
• Beobachtetes Molekulargewicht: 138-145 kDa
• GenBank-Zugangsnummer: BC015758
• Gene symbol: SKIV2L
• Gene ID (NCBI): 6499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-29-2025)

Specific detection of human Skiv2l in RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

SKIV2L Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:68547-1-Ig)

FH

Tsimafei (Verified Customer) (11-19-2024)

Antibody detect a band of the correct molecular weight in U2OS cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

SKIV2L Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:68547-1-Ig)