SKIV2L Monoklonaler Antikörper

SKIV2L Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A9G2

Kat-Nr. : 68547-1-PBS

Synonyme

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

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  WB analysis using 68547-1-Ig (same clone as 68547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68547-1-PBS bindet in WB, IHC, Indirect ELISA SKIV2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SKIV2L fusion protein Ag33679
• Vollständiger Name: superkiller viralicidic activity 2-like (S. cerevisiae)
• Berechnetes Molekulargewicht: 1246 aa, 138 kDa
• Beobachtetes Molekulargewicht: 138-145 kDa
• GenBank-Zugangsnummer: BC015758
• Gene symbol: SKIV2L
• Gene ID (NCBI): 6499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].