SKIV2L2 Rekombinanter Antikörper

SKIV2L2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251430D11

Kat-Nr. : 86536-1-RR

Synonyme

ATP dependent helicase SKIV2L2, ATP-dependent RNA helicase DOB1, ATP-dependent RNA helicase SKIV2L2, DOB1, EC:3.6.4.13

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  WB analysis using 86536-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86536-1-RR (SKIV2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86536-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIV2L2 antibody (86536-1-RR, Clone: 251430D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86536-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86536-1-RR against Human SKIV2L2 were performed. The affinity constant is 0.198 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maushodengewebe, Neuro-2a-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86536-1-RR bindet in WB, IF/ICC, ELISA SKIV2L2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SKIV2L2 fusion protein Ag3414
• Vollständiger Name: superkiller viralicidic activity 2-like 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 1042 aa, 118 kDa
• Beobachtetes Molekulargewicht: 118 kDa
• GenBank-Zugangsnummer: BC028604
• Gene symbol: SKIV2L2
• Gene ID (NCBI): 23517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SKIV2L2 (Ski2-like RNA helicase 2, also called Mtr4) is an evolutionarily conserved 3′→5′ RNA helicase belonging to the Ski2-like subfamily of DExH-box helicases.SKIV2L2 is an essential component of the nuclear exosome targeting (NEXT) complex, where it acts as the major ATP-dependent motor that threads RNA substrates into the catalytic core of the RNA exosome for processing and degradation. Through this activity, SKIV2L2 regulates the turnover of aberrant pre-mRNAs, non-coding RNAs, and promoter-associated RNAs, thereby influencing gene expression, ribosomal RNA biogenesis, and maintenance of nuclear RNA homeostasis. It plays a role in cell differentiation and spermatogenesis. Clinically, germline mutations in SKIV2L2 have been associated with tricho-hepato-enteric syndrome, highlighting its importance in intestinal and hepatic development. (PMID: 39863862)