SKP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SKP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15010-1-PBS

Synonyme

---

• 

  WB analysis using 15010-1-AP (same clone as 15010-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  WB result of SKP2 antibody (15010-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  WB result of SKP2 antibody (15010-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15010-1-AP (same clone as 15010-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  IP result of anti-SKP2 (IP:15010-1-AP, 4ug; Detection:15010-1-AP 1:300) with HEK-293 cells lysate 2440ug. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15010-1-AP (same clone as 15010-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (15010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

15010-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SKP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SKP2 fusion protein Ag6826
• Vollständiger Name: S-phase kinase-associated protein 2 (p45)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 46-48 kDa
• GenBank-Zugangsnummer: BC001441
• Gene symbol: SKP2
• Gene ID (NCBI): 6502
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).