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CD229/SLAMF3 Rekombinanter Antikörper

CD229/SLAMF3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242312E8

Kat-Nr. : 84817-4-PBS

Synonyme

Lymphocyte antigen 9, Ly-9, Ly9, Cell surface molecule Ly-9, CD229

Filter:
  • Western Blot (WB) analysis of EL-4 cells using CD229/SLAMF3 Recombinant antibody (84817-4-RR)

    WB analysis of EL-4 using 84817-4-RR (same clone as 84817-4-PBS)

    EL-4 cells were subjected to SDS PAGE followed by western blot with 84817-4-RR (CD229/SLAMF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84817-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD229/SLAMF3 Recombinant antibody (84817-4-RR)

    IHC staining of mouse spleen using 84817-4-RR (same clone as 84817-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84817-4-RR (SLAMF3/CD229 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84817-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84817-4-RR

    Affinity and Kinetic Characterization of 84817-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84817-4-RR against Mouse SLAMF3/CD229 were performed. The affinity constant is 2.04 nM.

Geprüfte Anwendungen

Produktinformation

84817-4-PBS bindet in WB, IHC, Indirect ELISA CD229/SLAMF3 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name lymphocyte antigen 9
Berechnetes Molekulargewicht73 kDa
Beobachtetes Molekulargewicht80-90 kDa, 110 kDa
GenBank-ZugangsnummerNM_001277968.1
Gene symbol Ly9
Gene ID (NCBI) 17085
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocytic activation molecule 3 (SLAMF3), also known as CD229 or Ly9, is a type I transmembrane glycoprotein that belongs to the SLAM family receptors (PMID: 10970093; 15905546). It is composed of an extracellular region, which consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail, which includes two immunoreceptor tyrosine-based signaling motifs (ITSMs) and other tyrosine residues that act as binding sites for the SH2 structural domains (PMID: 8537117; 11369645; 38626547). SLAMF3 is mainly expressed in immune cells, such as T, B, and natural killer cells, and it is involved in the fundamental activation and suppression of these immune cells, as well as the regulation of complex immune networks (PMID: 38626547).