CD229/SLAMF3 Rekombinanter Antikörper

CD229/SLAMF3 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242312E8

Kat-Nr. : 84817-4-RR

Synonyme

Lymphocyte antigen 9, Ly-9, Ly9, Cell surface molecule Ly-9, CD229

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  WB analysis of EL-4 using 84817-4-RR

  EL-4 cells were subjected to SDS PAGE followed by western blot with 84817-4-RR (CD229/SLAMF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84817-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84817-4-RR (SLAMF3/CD229 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84817-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84817-4-RR against Mouse SLAMF3/CD229 were performed. The affinity constant is 2.04 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EL-4 cells, Mausmilzgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84817-4-RR bindet in WB, IHC, ELISA CD229/SLAMF3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: lymphocyte antigen 9
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa, 110 kDa
• GenBank-Zugangsnummer: NM_001277968.1
• Gene symbol: Ly9
• Gene ID (NCBI): 17085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocytic activation molecule 3 (SLAMF3), also known as CD229 or Ly9, is a type I transmembrane glycoprotein that belongs to the SLAM family receptors (PMID: 10970093; 15905546). It is composed of an extracellular region, which consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail, which includes two immunoreceptor tyrosine-based signaling motifs (ITSMs) and other tyrosine residues that act as binding sites for the SH2 structural domains (PMID: 8537117; 11369645; 38626547). SLAMF3 is mainly expressed in immune cells, such as T, B, and natural killer cells, and it is involved in the fundamental activation and suppression of these immune cells, as well as the regulation of complex immune networks (PMID: 38626547).