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DMT1 Polyklonaler Antikörper

DMT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(113)

Kat-Nr. : 20507-1-AP

Synonyme

SLC11A2, Solute carrier family 11 member 2, OK/SW-cl.20, Natural resistance-associated macrophage protein 2, DMT-1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHuH-7-Zellen, Caco-2-Zellen, COLO 320-Zellen, Neuro-2a-Zellen, Rattennierengewebe, SH-SY5Y-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe, humanes Pankreasgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaus-Dünndarmgewebe, Maushirngewebe
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:400-1:1600
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20507-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA DMT1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DMT1 fusion protein Ag14314
Vollständiger Name solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Berechnetes Molekulargewicht 568 aa, 62 kDa
Beobachtetes Molekulargewicht 60-70 kDa
GenBank-ZugangsnummerBC002592
Gene symbol DMT1
Gene ID (NCBI) 4891
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that play a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including the brain, kidney, testis, duodenum, and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for DMT1 antibody 20507-1-APProtokoll herunterladen
IHC protocol for DMT1 antibody 20507-1-APProtokoll herunterladenl
IF protocol for DMT1 antibody 20507-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Blood

Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.

Authors - Floriane Petit
humanIHC

Acta Neuropathol

Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.

Authors - Till S Zimmer
mouseWB

Mol Cell

Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.

Authors - Ross A Weber
humanWB

Sci Adv

Magnetically attracting hydrogel reshapes iron metabolism for tissue repair

Authors - Yichang Xu
mouseWB

J Hazard Mater

Investigating the potential risk of cadmium exposure on seizure severity and anxiety-like behaviors through the ferroptosis pathway in epileptic mice: An integrated multi-omics approach

Authors - Yuanjin Chang
mouseIF

Nat Commun

Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.

Authors - Hirofumi Yamaguchi