NKCC1/SLC12A2 Polyklonaler Antikörper

NKCC1/SLC12A2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28055-1-AP

Synonyme

NKCC1,SLC12A2, NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter

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  WB analysis using 28055-1-AP

  Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28055-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NKCC1/SLC12A2 antibody (28055-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 28055-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NKCC1,SLC12A2 antibody (28055-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochte HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28055-1-AP bindet in WB, IHC, IF/ICC, ELISA NKCC1/SLC12A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKCC1/SLC12A2 fusion protein Ag27895
• Vollständiger Name: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC033003
• Gene symbol: NKCC1/SLC12A2
• Gene ID (NCBI): 6558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).