SLC12A8 Rekombinanter Antikörper

SLC12A8 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240082B11

Kat-Nr. : 83225-2-PBS

Synonyme

CCC9, Cation-chloride cotransporter 9, 240082B11

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  IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR (same clone as 83225-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83225-2-RR (SLC12A8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83225-2-RR (same clone as 83225-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC12A8 antibody (83225-2-RR, Clone: 240082B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83225-2-RR (same clone as 83225-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SLC12A8 Recombinant antibody (83225-2-RR, Clone:240082B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00171-1

  Cytometric bead array standard curve of MP00171-1, SLC12A8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83225-1-PBS. Detection antibody: 83225-2-PBS. Standard: Ag34354. Range: 5-40 ng/mL.

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  Affinity and Kinetic Characterization of 83225-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83225-2-RR against Human SLC12A8 were performed. The affinity constant is 3.03 nM.

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Geprüfte Anwendungen

Produktinformation

83225-2-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA SLC12A8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC12A8 fusion protein Ag34354
• Vollständiger Name: solute carrier family 12 (potassium/chloride transporters), member 8
• Berechnetes Molekulargewicht: 714 aa, 80 kDa
• GenBank-Zugangsnummer: BC020506
• Gene symbol: SLC12A8
• Gene ID (NCBI): 84561
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.