VGLUT1 Polyklonaler Antikörper

VGLUT1 Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 55491-1-AP

Synonyme

1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter

---

• 

  WB analysis using 55491-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55491-1-AP (VGLUT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 55491-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT1 antibody (55491-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 55491-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT1 antibody (55491-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 55491-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human VGLUT1 (55491-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochtes Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:750-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 18 publications below

Produktinformation

55491-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA VGLUT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: NM_020309
• Gene symbol: VGLUT1
• Gene ID (NCBI): 57030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons.

Publikationen

Species	Application	Title
mouse	IF	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse	IF	PLoS Biol; The anterior cingulate cortex controls the hyperactivity in subthalamic neurons in male mice with comorbid chronic pain and depression; Authors - Ying-Di Wang
human	IF	iScience; Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons; Authors - Waka Lin
human	IF	Neurobiol Dis; GRN-/- iPSC-derived cortical neurons recapitulate the pathological findings of both frontotemporal lobar degeneration and neuronal ceroidolipofuscinosis; Authors - Patrizia Bossolasco
mouse	IF	J Neurosci; Insula→Amygdala and Insula→Thalamus Pathways Are Involved in Comorbid Chronic Pain and Depression-Like Behavior in Mice; Authors - Jing Chen
rat	IF	Neurobiol Dis; PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by influencing synaptic function in the primary motor cortex of rats.; Authors - Juan Mo