SLC17A9 Rekombinanter Antikörper

SLC17A9 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240328H7

Kat-Nr. : 83505-1-PBS

Synonyme

Voltage-gated purine nucleotide uniporter SLC17A9, VNUT, Vesicular nucleotide transporter, FLJ23412, C20orf59

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  WB analysis using 83505-1-RR (same clone as 83505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83505-1-RR (SLC17A9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 83505-1-RR (same clone as 83505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 83505-1-RR (same clone as 83505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83505-1-RR (same clone as 83505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  FC experiment of Caco-2 using 83505-1-RR (same clone as 83505-1-PBS)

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC17A9 Recombinant antibody (83505-1-RR, Clone:240328H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83505-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83505-1-RR against Human SLC17A9 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83505-1-PBS bindet in WB, IHC, FC (Intra), ELISA SLC17A9 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC17A9 fusion protein Ag24970
• Vollständiger Name: solute carrier family 17, member 9
• Berechnetes Molekulargewicht: 436 aa, 47 kDa
• Beobachtetes Molekulargewicht: 50 kD
• GenBank-Zugangsnummer: BC025312
• Gene symbol: SLC17A9
• Gene ID (NCBI): 63910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.