EAAT3 Polyklonaler Antikörper

EAAT3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12686-1-AP

Synonyme

EAAC1, EAAT3, SLC1A1

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Galerie der Validierungsdaten

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  WB analysis of mouse ovary using 12686-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12686-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12686-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12686-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, humanes Hirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

12686-1-AP bindet in WB, IHC, ELISA EAAT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EAAT3 fusion protein Ag3379
• Vollständiger Name: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
• Berechnetes Molekulargewicht: 524 aa, 57 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC033040
• Gene symbol: SLC1A1
• Gene ID (NCBI): 6505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Cancer Res; Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer.; Authors - Wenzheng Guo
mouse	WB	Neuropharmacology; Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease.; Authors - Yun-Long Zhang
mouse	WB	J Alzheimers Dis; S-Adenosylmethionine Alleviates Amyloid-β-Induced Neural Injury by Enhancing Trans-Sulfuration Pathway Activity in Astrocytes.; Authors - Xinkun Wan
goat	IHC	Anim Sci J; The expression of nutrient chemosensing gate molecules in the ileum and colon is altered for goats fed on a high-grain diet; Authors - Changxin Tian
mouse	WB	Nutrients; L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway; Authors - Kehong Liu