EAAT4 Polyklonaler Antikörper

EAAT4 Polyklonal Antikörper für WB, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 12876-1-AP

Synonyme

SLC1A6, Sodium-dependent glutamate/aspartate transporter

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  WB analysis of A375 using 12876-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12876-1-AP (EAAT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse cerebellum using 12876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse cerebellum using 12876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen
• Erfolgreiche Detektion in IF-Fro: Maus-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

12876-1-AP bindet in WB, IF-Fro, ELISA EAAT4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EAAT4 fusion protein Ag3554
• Vollständiger Name: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
• Berechnetes Molekulargewicht: 61.6 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC028721
• Gene symbol: EAAT4
• Gene ID (NCBI): 6511
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excitatory amino acid transporter 4 (EAAT4) is a neuronal glutamate transporter responsible for the reuptake of synaptically released glutamate. The EAAT4 protein is highly enriched in Purkinje cells of the cerebellum, although it is not restricted to these cells (18770868). While the predicted molecular weight of EAAT4 is 62 kDa, considerable heterogeneity in transporter size has been reported in the brain with molecular weights ranging from 50 to 120 kDa as a result of differential glycosylation and/or phosphorylation (24056007). This antibody recognizes 60-70 kDa of EAAT4 in mouse brain and several other lysate.

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell Firing Pattern of NPC1-deficient Mice.; Authors - Michael Rabenstein
human	WB	Neoplasia; RUNX2 enhances bladder cancer progression by promoting glutamine metabolism; Authors - Zhigang Huang
human	WB	Cancer Immunol Immunother; Glutamate transporter SLC1A6 promotes resistance to immunotherapy in cancer; Authors - Chenchen Li