SLC20A2 Polyklonaler Antikörper

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• KD/KO Validated

SLC20A2 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 12820-1-AP

Synonyme

Pit2, Phosphate transporter 2, hPit2, GLVR-2, GLVR2

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  WB analysis using 12820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12820-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 12820-1-AP

  IP result of anti-SLC20A2 (IP:12820-1-AP, 4ug; Detection:12820-1-AP 1:400) with COLO 320 cells lysate 2400ug.

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  IHC staining of mouse testis using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 12820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC20A2 antibody (12820-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, COLO 320-Zellen, Mausnierengewebe
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: human bowen disease, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

12820-1-AP bindet in WB, IHC, IF-P, IP, ELISA SLC20A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC20A2 fusion protein Ag3469
• Vollständiger Name: solute carrier family 20 (phosphate transporter), member 2
• Berechnetes Molekulargewicht: 652 aa, 70 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC028600
• Gene symbol: SLC20A2
• Gene ID (NCBI): 6575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC20A2 (also known as PiT2) is a sodium (Na)-phosphate (Pi) co-transporter that belongs to the SLC20 family. SLC20A2 is highly expressed in the central nervous system and controls hippocampal-dependent learning and memory (PMID: 38195526). SLC20A2 is the major causative gene for primary familial brain calcification (PFBC), a rare neurological disease characterized by widespread cognitive impairment.

Publikationen

Species	Application	Title
human	WB	Adv Healthc Mater; Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds; Authors - Wei Chen ; KD Validated
mouse	WB	Br J Pharmacol; Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.; Authors - Katalin Éva Sikura
		Endocrinology; Evocalcet rescues secondary hyperparathyroidism-driven cortical porosity in chronic kidney disease male rats; Authors - Tomoka Hasegawa
mouse	WB	Bone; Growth-related skeletal changes and alterations in phosphate metabolism.; Authors - Toshimi Michigami
human	WB	J Biol Chem; Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake.; Authors - Nina Bon
human	WB	Arterioscler Thromb Vasc Biol; Potential Role of H-Ferritin in Mitigating Valvular Mineralization.; Authors - Katalin Éva Sikura