URAT1 Polyklonaler Antikörper

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URAT1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(93)

Kat-Nr. : 14937-1-AP

Synonyme

SLC22A12, RST, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein

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  WB analysis using 14937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 14937-1-AP

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe, Hausschwein-Nierengewebe, Rattenherzgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 85 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Produktinformation

14937-1-AP bindet in WB, IHC, IF, ELISA URAT1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: URAT1 fusion protein Ag6770
• Vollständiger Name: solute carrier family 22 (organic anion/urate transporter), member 12
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa, 40 kDa
• GenBank-Zugangsnummer: BC053348
• Gene symbol: URAT1
• Gene ID (NCBI): 116085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.

Publikationen

Species	Application	Title
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo
human,mouse	WB	Phytomedicine; The inhibitory effect of Astragalus flavone extract on hyperuricemia and its underlying molecular mechanism by targeting JNK/AP-1/NLRP3/IL-1β signaling pathway; Authors - Hongjuan Xia
rat	WB	Phytomedicine; Mechanistic insights into the potentiation and toxicity mitigation of myocardial infarction treatment with salvianolate and ticagrelor; Authors - Yan Wan
mouse	WB	Mol Metab; URAT1-selective inhibition ameliorates insulin resistance by attenuating diet-induced hepatic steatosis and brown adipose tissue whitening in mice.; Authors - Yoshiro Tanaka
mouse	IHC	Food Funct; Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice; Authors - Arshad Mehmood
mouse	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang