SLC24A6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC24A6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 21430-1-AP

Synonyme

NCKX6, NCLX, SLC24A6, SLC8B1

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Galerie der Validierungsdaten

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  WB analysis using 21430-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

21430-1-AP bindet in WB, IHC, IF, ELISA SLC24A6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC24A6 fusion protein Ag16034
• Vollständiger Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 6
• Berechnetes Molekulargewicht: 584 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC098360
• Gene symbol: SLC24A6
• Gene ID (NCBI): 80024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC24A6, also named as NCLX, is a kind of mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion by mediating the exchange of 3 sodium ions per 1 calcium ion. It has been reported that SLC24A6 plays a key role in attenuating potential ROS production and injury upon cardiac reperfusion. SLC24A6 is associated with mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion. (PMID: 32728214, 20018762, 28219928)

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
mouse	WB	J Cardiovasc Transl Res; CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes.; Authors - Yao Xu
human	WB	Oncol Lett; Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX; Authors - Jiang Chang
rat	WB,IF	Cell Biochem Biophys; Astragalus Polysaccharides Reduce High-glucose-induced Rat Aortic Endothelial Cell Senescence and Inflammasome Activation by Modulating the Mitochondrial Na+/Ca2+ Exchanger.; Authors - Xin-Yu Miao
rat	WB, IF	J Geriatr Cardiol; The mitochondrial Na(+)/Ca(2+) exchanger may reduce high glucose-induced oxidative stress and nucleotide-binding oligomerization domain receptor 3 inflammasome activation in endothelial cells.; Authors - Yuan Zu ; KD Validated
human	IHC,IF	Cell Death Dis; Lon upregulation contributes to cisplatin resistance by triggering NCLX-mediated mitochondrial Ca2+ release in cancer cells.; Authors - Vidhya Tangeda