SLC25A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC25A1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Rezensionen (2)

Kat-Nr. : 15235-1-AP

Synonyme

CIC, Citrate transport protein, CTP, SLC20A3, Tricarboxylate carrier protein

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  WB analysis using 15235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15235-1-AP (SLC25A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15235-1-AP

  WB result of SLC25A1 antibody (15235-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A1 transfected HeLa cells.

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  WB analysis of rat liver using 15235-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15235-1-AP (SLC25A1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15235-1-AP

  IP result of anti-SLC25A1 (IP:15235-1-AP, 4ug; Detection:15235-1-AP 1:5000) with HeLa cells lysate 1400 ug.

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  IHC staining of human breast cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15235-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15235-1-AP (SLC25A1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15235-1-AP

  Immunofluorescent analysis of HepG2 cells using 15235-1-AP (SLC25A1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, NCI-H1299-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 32 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

15235-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SLC25A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A1 fusion protein Ag7352
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC004980
• Gene symbol: SLC25A1
• Gene ID (NCBI): 6576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

Publikationen

Species	Application	Title
mouse	WB	Nature; A non-canonical tricarboxylic acid cycle underlies cellular identity.; Authors - Paige K Arnold
		Circulation; Glycolytic Switch Is Required for Transdifferentiation to Endothelial Lineage.; Authors - Li Lai
mouse	WB,IP	Nat Commun; IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.; Authors - Weiwei Liu
mouse	WB	EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human	WB	Am J Hum Genet; Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.; Authors - Benjamin Nota
mouse,human	WB,IHC	Cell Death Differ; Inhibition of the mitochondrial citrate carrier, Slc25a1, reverts steatosis, glucose intolerance, and inflammation in preclinical models of NAFLD/NASH.; Authors - Mingjun Tan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bruna (Verified Customer) (03-05-2022)

This ab works very well for WB in human and mouse cell lines. Very strong and specific band (tested with KO cells lines).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs/U2OS

FH

Chunling (Verified Customer) (12-06-2018)

This antibody works great for western blot and Immunofluorescence. It is very specific with no non specific bands in western.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: kidney cancer cells