SLC25A12 Polyklonaler Antikörper

SLC25A12 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26804-1-PBS

Synonyme

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

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  WB analysis using 26804-1-AP (same clone as 26804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 26804-1-AP (same clone as 26804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 26804-1-AP (same clone as 26804-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed tissue using 26804-1-AP (SLC25A12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26804-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA SLC25A12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A12 fusion protein Ag25150
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Berechnetes Molekulargewicht: 75 kDa, 63 kDa
• Beobachtetes Molekulargewicht: ~70 kDa
• GenBank-Zugangsnummer: BC016932
• Gene symbol: SLC25A12
• Gene ID (NCBI): 8604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).