SLC25A12 Monoklonaler Antikörper

SLC25A12 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2B10

Kat-Nr. : 67467-1-Ig

Synonyme

AGC1, ARALAR, ARALAR1, SLC25A12

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Galerie der Validierungsdaten

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  WB analysis of LNCaP using 67467-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67467-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 67467-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A431-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Mammakarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67467-1-Ig bindet in WB, IHC,ELISA SLC25A12 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLC25A12 fusion protein Ag13576
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Berechnetes Molekulargewicht: 75 kDa, 63 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC016932
• Gene symbol: SLC25A12
• Gene ID (NCBI): 8604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.