SLC25A13 Polyklonaler Antikörper

SLC25A13 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10789-1-PBS

Synonyme

Mitochondrial aspartate glutamate carrier 2, Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial, Citrin, Calcium-binding mitochondrial carrier protein Aralar2, ARALAR-related gene 2

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  WB analysis of mouse kidney using 10789-1-AP (same clone as 10789-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  WB analysis using 10789-1-AP (same clone as 10789-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10789-1-AP (same clone as 10789-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10789-1-AP (same clone as 10789-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 10789-1-AP (same clone as 10789-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 10789-1-AP (same clone as 10789-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 10789-1-AP (same clone as 10789-1-PBS)

  IP result of anti-SLC25A13 (IP:10789-1-AP, 4ug; Detection:10789-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10789-1-AP (same clone as 10789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10789-1-AP (same clone as 10789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 10789-1-AP (same clone as 10789-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC25A13 antibody (10789-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10789-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SLC25A13 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A13 fusion protein Ag1242
• Vollständiger Name: solute carrier family 25, member 13 (citrin)
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 60-63 kDa
• GenBank-Zugangsnummer: BC006566
• Gene symbol: SLC25A13
• Gene ID (NCBI): 10165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A13, also known as citrin, has been identified as a mitochondrial aspartate-glutamate carrier protein and may be implicated in metabolic compartmentation. It consists of 675 amino acid residues and harbors four EF-hands and six mitochondrial transmembranous (TM) spanners. Mutations in SLC25A13 gene cause adult-onset type II citrullinemia (CTLN2) and neonatal intrahepatic cholestasis (NICCD). The complete deletion or cleaved SLA25A13 proteins have been reported in patients with SLC25A13 mutation.